Accelerated solvent extraction (ASE) was used for the extraction of carotenoids from pre-treated biomass of Dunaliella salina microalgae. The total carotenes and lutein were extracted using different solvents i.e., acetone, chloroform: methanol (C:M) in the ratio of 1:1, ethanol, and hexane. The effects of various experimental parameters, such as temperature (20, 40, 60 and 80 °C), pressure (50 and 100 bar) and static time (2 cycles of 5 and 10 minutes) on the ASE extraction efficiency were investigated systematically. The properties of the solvent and the extraction temperature were found to be the most important parameters affecting the ASE extraction efficiency of thermolabile natural compounds. Moreover, 99% recovery of total carotenes was achieved during extraction with hexane solvent at 40 °C, 100 bar and 2 cycle of 10 minutes. While solvent mixture of C:M was suitable for lutein extraction and resulted 92% recovery at 20 °C, 100 bar and 2 cycle of 10 minutes.

Extraction of carotenes and lutein from dunaliella salina microalgae using pressurized liquid extraction

Sharma, N.;Mehariya, S.;Martino, M.;Larocca, V.;Di Sanzo, G.;Balducchi, R.;Molino, A.
2019

Abstract

Accelerated solvent extraction (ASE) was used for the extraction of carotenoids from pre-treated biomass of Dunaliella salina microalgae. The total carotenes and lutein were extracted using different solvents i.e., acetone, chloroform: methanol (C:M) in the ratio of 1:1, ethanol, and hexane. The effects of various experimental parameters, such as temperature (20, 40, 60 and 80 °C), pressure (50 and 100 bar) and static time (2 cycles of 5 and 10 minutes) on the ASE extraction efficiency were investigated systematically. The properties of the solvent and the extraction temperature were found to be the most important parameters affecting the ASE extraction efficiency of thermolabile natural compounds. Moreover, 99% recovery of total carotenes was achieved during extraction with hexane solvent at 40 °C, 100 bar and 2 cycle of 10 minutes. While solvent mixture of C:M was suitable for lutein extraction and resulted 92% recovery at 20 °C, 100 bar and 2 cycle of 10 minutes.
Accelerated solvent extraction, Dunaliella salina, GRAS solvents, Microalgae, Natural carotenoids
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12079/54761
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