The work target was to compare the efficiency of two hydrothermal pretreatments, viz.: Steam Explosion (SE) and Liquid Hot Water (LHW), towards fractionation of pretreated wheat straw and enzymatic digestibility of the cellulosic residue. The SE and LHW were performed at two severities with and without acidic catalyst (H2SO4, 3%). The SE was performed with a batch reactor of 10 L, coupled with a 125 kW boiler. The LHW was carried out with a high pressure and temperature batch reactor (PARR) of a 0.270 l vessel with adjustable internal stirrer and heat control. After SE and LHW pretreatments the materials were extracted by water to remove and quantify the soluble hemicellulose as monomers and oligomers as well the inhibitors; the solid residue was saccharified by enzymatic hydrolysis (EH) at the solids loading of 5% (w/v), 50 °C and pH 4.8 for 72h with a stirring speed of 180 rpm. The residual lignin was determined gravimetrically. As general trend, the digestibility of cellulose was slightly higher with the SE, while LHW allowed higher hemicellulosic sugars recovery in the aqueous fraction.
Sugar production from wheat straw by hydrothermal pretreatments and enzymatic hydrolysis
Viola, E.;Zimbardi, F.;Cerone, N.;Romanelli, A.;Valerio, V.;
2019-01-01
Abstract
The work target was to compare the efficiency of two hydrothermal pretreatments, viz.: Steam Explosion (SE) and Liquid Hot Water (LHW), towards fractionation of pretreated wheat straw and enzymatic digestibility of the cellulosic residue. The SE and LHW were performed at two severities with and without acidic catalyst (H2SO4, 3%). The SE was performed with a batch reactor of 10 L, coupled with a 125 kW boiler. The LHW was carried out with a high pressure and temperature batch reactor (PARR) of a 0.270 l vessel with adjustable internal stirrer and heat control. After SE and LHW pretreatments the materials were extracted by water to remove and quantify the soluble hemicellulose as monomers and oligomers as well the inhibitors; the solid residue was saccharified by enzymatic hydrolysis (EH) at the solids loading of 5% (w/v), 50 °C and pH 4.8 for 72h with a stirring speed of 180 rpm. The residual lignin was determined gravimetrically. As general trend, the digestibility of cellulose was slightly higher with the SE, while LHW allowed higher hemicellulosic sugars recovery in the aqueous fraction.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
