A reliable and reproducible genotype-independent protocol for slow-growth storage of globe artichoke was established for the first time to meet two needs: genetic resources conservation and labour costs reduction in commercial laboratories. Growth reduction was achieved by supplementing osmotic agents to the media. Plant responses to in vitro storage, genetic stability and field performance were the parameters used to evaluate the germplasm conservation conditions. Forty-nine treatments were applied, as the result of seven genotypes with seven media. After 12 months of storage, culture survival across genotypes ranged from 65% to 85% and all the media tested supported 100% regrowth. All genotypes regained their full growth potential within two months. Genetic stability between mother plants grown in the field and in vitro conserved plantlets at 6 and 12 months of storage was assessed by molecular markers to identify the most suitable storage media.Protocol suitability was validated by a field test using an approved list of plant descriptors for globe artichoke. Morphological data highlighted that the slight genetic instability detected by molecular markers did not affect significantly plants morphology and their agronomic traits.The results indicate that the minimal growth medium of choice for globe artichoke conservation is the one where the seven genotypes displayed phenotypes similar to the control, coupled with the lowest percentage of changes (2.43%) at a molecular level. As far as we know there are no published reports on in vitro conservation protocolfor globe artichoke applied to different genotypes and validated by an appropriate field test. © 2015 Elsevier B.V.
A validated slow-growth in vitro conservation protocol for globe artichoke germplasm: A cost-effective tool to preserve from wild to elite genotypes
Tavazza, R.
2015-01-01
Abstract
A reliable and reproducible genotype-independent protocol for slow-growth storage of globe artichoke was established for the first time to meet two needs: genetic resources conservation and labour costs reduction in commercial laboratories. Growth reduction was achieved by supplementing osmotic agents to the media. Plant responses to in vitro storage, genetic stability and field performance were the parameters used to evaluate the germplasm conservation conditions. Forty-nine treatments were applied, as the result of seven genotypes with seven media. After 12 months of storage, culture survival across genotypes ranged from 65% to 85% and all the media tested supported 100% regrowth. All genotypes regained their full growth potential within two months. Genetic stability between mother plants grown in the field and in vitro conserved plantlets at 6 and 12 months of storage was assessed by molecular markers to identify the most suitable storage media.Protocol suitability was validated by a field test using an approved list of plant descriptors for globe artichoke. Morphological data highlighted that the slight genetic instability detected by molecular markers did not affect significantly plants morphology and their agronomic traits.The results indicate that the minimal growth medium of choice for globe artichoke conservation is the one where the seven genotypes displayed phenotypes similar to the control, coupled with the lowest percentage of changes (2.43%) at a molecular level. As far as we know there are no published reports on in vitro conservation protocolfor globe artichoke applied to different genotypes and validated by an appropriate field test. © 2015 Elsevier B.V.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
