EXPRESSION of viral genes in transgenic plants is a very effective tool for attenuating plant viral infection1–3. Nevertheless, the lack of generality and risk issues related to the expression of viral genes in plants4 might limit the exploitation of this strategy. Expression in plants of antibodies against essential viral proteins could provide an alternative approach to engineer viral resistance. Recently, expression of complete5–7 or engineered7–9 antibodies has been successfully achieved in plants. The engineered single-chain Fv antbody scFv (refs 10,11) is particularly suitable for expression in plants because of its small size and the lack of assembly requirements. Here we present evidence that constitutive expression in transgenic plants of a scFv antibody, directed against the plant icosahedral tombusvirus artichoke mottled crinkle virus, causes reduction of infection incidence and delay in symptom development.

Transgenic plants expressing functional "Single Chain Fv" antibody are specifically protected from virus attack

Galeffi, Patrizia;De Martinis, Domenico;Benvenuto, Eugenio;
1993

Abstract

EXPRESSION of viral genes in transgenic plants is a very effective tool for attenuating plant viral infection1–3. Nevertheless, the lack of generality and risk issues related to the expression of viral genes in plants4 might limit the exploitation of this strategy. Expression in plants of antibodies against essential viral proteins could provide an alternative approach to engineer viral resistance. Recently, expression of complete5–7 or engineered7–9 antibodies has been successfully achieved in plants. The engineered single-chain Fv antbody scFv (refs 10,11) is particularly suitable for expression in plants because of its small size and the lack of assembly requirements. Here we present evidence that constitutive expression in transgenic plants of a scFv antibody, directed against the plant icosahedral tombusvirus artichoke mottled crinkle virus, causes reduction of infection incidence and delay in symptom development.
Virus;Molecular biology;Plant;GMO;Genetic engineering;Biochemistry;Biochemistry;Antibody;Plant;Biology
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12079/428
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
social impact