Microalgae Dunaliella salina contains useful molecules such as β-carotene and fatty acids (FAs), which are considered high value-added compounds. To extract these molecules, supercritical carbon dioxide was used at different operative conditions. The effects of mechanical pre-treatment (grinding speed at 0–600 rpm; pre-treatment time of 2.5–7.5 min) and operating parameters for extraction, such as biomass loading (2.45 and 7.53 g), pressure (100–550 bars), temperature (50–75◦C) and CO2 flow rate (7.24 and 14.48 g/min) by varying the extraction times (30–110 min) were evaluated. Results showed that the maximum cumulative recovery (25.48%) of β-carotene was achieved at 400 bars and 65◦C with a CO2 flow rate of 14.48 g/min, while the highest purity for stage (55.40%) was attained at 550 bars and 65◦C with a CO2 flow rate of 14.48 g/min. The maximum recovery of FAs, equal to 8.47 mg/g, was achieved at 550 bars and 75◦C with a CO2 flow rate of 14.48 g/min. Moreover, the lowest biomass loading (2.45 g) and the first extraction cycle (30 min) allowed the maximum extraction of β-carotene and FAs.

Extraction of bioactive compounds using supercritical carbon dioxide

Molino A.;Larocca V.;Di Sanzo G.;Martino M.;Casella P.;
2019

Abstract

Microalgae Dunaliella salina contains useful molecules such as β-carotene and fatty acids (FAs), which are considered high value-added compounds. To extract these molecules, supercritical carbon dioxide was used at different operative conditions. The effects of mechanical pre-treatment (grinding speed at 0–600 rpm; pre-treatment time of 2.5–7.5 min) and operating parameters for extraction, such as biomass loading (2.45 and 7.53 g), pressure (100–550 bars), temperature (50–75◦C) and CO2 flow rate (7.24 and 14.48 g/min) by varying the extraction times (30–110 min) were evaluated. Results showed that the maximum cumulative recovery (25.48%) of β-carotene was achieved at 400 bars and 65◦C with a CO2 flow rate of 14.48 g/min, while the highest purity for stage (55.40%) was attained at 550 bars and 65◦C with a CO2 flow rate of 14.48 g/min. The maximum recovery of FAs, equal to 8.47 mg/g, was achieved at 550 bars and 75◦C with a CO2 flow rate of 14.48 g/min. Moreover, the lowest biomass loading (2.45 g) and the first extraction cycle (30 min) allowed the maximum extraction of β-carotene and FAs.
Dunaliella salina; Fatty acids; Food additives; Microalgae; Supercritical-CO; 2; fluid extraction; β-carotene; Carbon Dioxide; Diatomaceous Earth; Fatty Acids; Humans; Liquid-Liquid Extraction; Microalgae; Pressure; Rheology; Solid Phase Extraction; Temperature; Time Factors; beta Carotene
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.12079/52756
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