The flower market of the Terlizzi agro-industrial district (Bari, Italy) represents the 20% of the Italian production, which is the second in Europe after the Netherlands. The main products are cut flowers and plants in vase, in particular roses and cyclamens. In this framework the European Project "Ecoflower Terlizzi" was carried out to support the implementation of a sustainable environmental policy in the Terlizzi district. The project was aimed at defining the criteria of environmental quality of the flowers produced in this area and includes both the definition of an EPD (Environmental Product Declaration) programme and of a local eco-label (type I). To reach this objective environmental analyses and LCA (Life Cycle Assessment) studies were carried out on a sample of seven enterprises representative of the Terlizzi's production systems. They were analysed on the basis of on-site data collection including materials and components for structures and equipments, energy and water consumption, fertilizers, pesticides and other chemicals used in the cultivation phase. Three farms produce roses with soilless cultivation systems, two produce roses in soil and two produce cyclamens' pots. In this paper the main issues concerning the application of the LCA methodology to the production of roses and cyclamens and the results obtained will be presented. Thermal energy consumption gives the main contribution to the environmental impacts of the roses' life cycle. For the cyclamens' pots the main contribution comes from the seedlings production. For both productions, electricity consumption, structures and equipments give a significant contribution to the environmental impacts.