Embryo manipulation is a requisite step in assisted reproductive technology (ART). There-fore, it is of great necessity to appraise the safety of ART and investigate the long-term effect, including lipid metabolism, on ART-conceived offspring. Augmenting our ART rabbit model to investigate lipid metabolic outcomes in offspring longitudinally, we detected variations in hepatic DNA methy-lation ART offspring in the F3 generation for embryonic exposure (multiple ovulation, vitrification and embryo transfer). Through adult liver metabolomics and proteomics, we identified changes mainly related to lipid metabolism (e.g., polyunsaturated fatty acids, steroids, steroid hormone). We also found that DNA methylation analysis was linked to changes in lipid metabolism and apoptosis genes. Nevertheless, these differences did not apparently alter the general health status. Thus, our findings suggest that ART is likely to be a player in embryo epigenetic events related to hepatic homeostasis alteration in adulthood.

Early embryo exposure to assisted reproductive manipulation induced subtle changes in liver epigenetics with no apparent negative health consequences in rabbit

Diretto G.;Frusciante S.;
2021-01-01

Abstract

Embryo manipulation is a requisite step in assisted reproductive technology (ART). There-fore, it is of great necessity to appraise the safety of ART and investigate the long-term effect, including lipid metabolism, on ART-conceived offspring. Augmenting our ART rabbit model to investigate lipid metabolic outcomes in offspring longitudinally, we detected variations in hepatic DNA methy-lation ART offspring in the F3 generation for embryonic exposure (multiple ovulation, vitrification and embryo transfer). Through adult liver metabolomics and proteomics, we identified changes mainly related to lipid metabolism (e.g., polyunsaturated fatty acids, steroids, steroid hormone). We also found that DNA methylation analysis was linked to changes in lipid metabolism and apoptosis genes. Nevertheless, these differences did not apparently alter the general health status. Thus, our findings suggest that ART is likely to be a player in embryo epigenetic events related to hepatic homeostasis alteration in adulthood.
2021
Assisted reproductive technologies
DNA methylation
Metabolome
Proteome
Steroid biosynthesis
Stress
Vitrification
Animals
Embryo, Mammalian
Epigenesis, Genetic
Female
Genome
Humans
Lipid Metabolism
Liver
Male
Metabolome
Pregnancy
Pregnancy, Animal
Proteome
Proteomics
Rabbits
Reproduction
Steroids
Vitrification
DNA Methylation
Embryo Transfer
Epigenomics
Reproductive Techniques, Assisted
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12079/62441
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