In this contribution we present results from the first N2 seeding experiments in JET performed after installation of the ITER-like Wall. Gas balance measurements for seeded L-mode discharges indicate very strong N2 retention as well as a potential increase in D2 retention. The possible influence of ammonia production on this apparent retention is discussed. Plasma parameters and impurity content were monitored throughout the seeded discharges as well as during subsequent clean-up discharges. These experiments give first insight into phenomena related to the use of nitrogen as seeding gas in JET with the ITER-like Wall, such as ammonia production and nitrogen legacy. © 2013 Elsevier B.V. All rights reserved.